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KMID : 0545120180280020267
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 2 p.267 ~ p.274
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Enhanced Production of Fatty Acids via Redirection of Carbon Flux in Marine Microalga Tetraselmis sp.
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Han Mi-Ae
Hong Seong-Joo
Kim Z-Hun
Cho Byung-Kwan
Lee Hoo-Keun
Choi Hyung-Kyoon
Lee Choul-Gyun
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Abstract
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Lipids in microalgae are energy-rich compounds and considered as an attractive feedstock for biodiesel production. To redirect carbon flux from competing pathways to the fatty acid synthesis pathway of Tetraselmis sp., we used three types of chemical inhibitors that can block the starch synthesis pathway or photorespiration, under nitrogen-sufficient and nitrogendeficient conditions. The starch synthesis pathway in chloroplasts and the cytosol can be inhibited by 3-(3,4-dichlorophenyl)-1,1-dimethylurea and 1,2-cyclohexane diamine tetraacetic acid (CDTA), respectively. Degradation of glycine into ammonia during photorespiration was blocked by aminooxyacetate (AOA) to maintain biomass concentration. Inhibition of starch synthesis pathways in the cytosol by CDTA increased fatty acid productivity by 27% under nitrogen deficiency, whereas the blocking of photorespiration in mitochondria by AOA was increased by 35% under nitrogen-sufficient conditions. The results of this study indicate that blocking starch or photorespiration pathways may redirect the carbon flux to fatty acid synthesis.
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KEYWORD
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Tetraselmis sp., fatty acids, carbohydrates, nitrogen starvation, chemical inhibitors
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